The four people killed near Wawa after two vehicles collided Sunday night were all from Western Canada.
OPP say two people in a southbound vehicle died at the scene when they hit a moose, crossed the highway and crashed into a northbound vehicle.
The victims in the southbound vehicle were a 45-year-old man and 14-year-old boy.
A 51-year-old woman and nine-year-old girl, both from Winnipeg and in the other vehicle, died in hospital.
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Five other people in both vehicles were injured and OPP are continuing their investigation.
The Sunday night crash closed the Trans-Canada Highway in both directions until 1:00pm Monday.